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Innovation

Originality

Imagination

 

Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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PCR Reaction Calculator

Novagen/Toyobo KOD hotstart polymerase

Reactions:

 ComponentVolume:
SingleFinal
10X Buffer 5.00 50.00
mM dNTPs 5.00 50.00
25mM MgSO4( mM final) 3.00 30.00
Forward Primer ( µM) 1.50 15.00
Reverse Primer ( µM) 1.50 15.00
Template 10.00
1.00 10.00
ddH2O 33.00 330.00
500.00

 

Recommended cycling parameters:

Use 1-2mM MgSO4 (1.5 is standard (used to be one, but protocol changed), 2 may work better for genomic template).

  • Activate the enzyme by incubating at 94°C for 2 min.
  • Perform 30-40 cycles:
    • Denature 15 sec at 94°C
    • Anneal 30 sec at primer Tm
    • Extend 68°C for 1 min per kb.
  • Finish with 5 min at 68°C