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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Bleaching C. elegans

  • Wash worms off a plate with cold M9.
  • Wash away the bacteria.
  • Add 1 ml of bleach solution.
  • Vortex for 2 min.
  • Spin down, remove old bleach and add new bleach.
  • Vortex again. Check periodically untill most of the worms have dissolved.
  • Wash 2x with M9 and put on a plate or in S Medium.

 

Bleach:

5 ml50 ml100 ml 
125 ┬Ál 1.25 ml 2.5 ml 10M NaOH
1.2 ml 12 ml 24 ml 5.25% Bleach
3.7 ml 36.75 ml 73.5 ml H2O