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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Freezing Worms

  1. Resuspend worms in S-basal or S-medium.
  2. Add 1 volume of freezing solution.
  3. Freeze slowly to -80°C

Freezing solution

Mix the following:

NaCl 5.85 g
KH2PO4 6.8 g
Glycerol 300 g
1M NaOH 5.6 ml
H2O to 1L

Autoclave, then add 3 ml of 0.1 M MgSO4