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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Single worm lysis and PCR

  • Prefill PCR tubes with 5 µl lysis buffer without proteinase K. Strips of PCR tubes work well.
  • Pick single worms to a fresh plate without food. When the worm crawls away from the bit of food transferred with the worm, use a pipette with a 10µl tip to put a drop of 2 µl of lysis buffer on the worm. Then pipette the worm off from the plate and into the prefilled PCR tube.
  • When all of the worms have been picked, quick freeze the tube in liquid N2, and thaw.
  • Add 5 µl lysis buffer containing proteinase K
  • Incubate 1 hour at 60°C to lyse the worm
  • Incubate 15 min. at 95°C to inactivate proteinase K
  • Use 2 µl in a PCR reaction
Worm lysis buffer
KCl 50 mM
Tris-HCl pH 8.3 10 mM
MgCl2 2.5 mM
NP-40 0.45%
Tween 20 0.45%

Just before use, add 6 µl of 10 mg/ml proteinase K to 0.5 ml of lysis mix.