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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Bacterial Media

SOB medium

Per liter:

bacto tryptone 20 g
bacto yeast extract 5 g
NaCl 0.5 g
KCl 0.186 g


Adjust to pH 7.0 with NaOH if necessary.

Just before use, add 5 ml of 2M MgCl2.

SOC medium

To 1L of SOB medium, add 5 ml of 40% glucose. 

LB medium

Per liter:

bacto tryptone 10 g
yeast extract 5 g
NaCl 5 g

Adjust to pH 7.0 with NaOH if necessary.

TB (Terrific Broth) medium

Autoclave separate solutions of medium and KPO4:

TB medium:

Tryptone 12 g
Yeast extract 24 g
Glycerol 4 ml
H2O to 900 ml

KPO4 buffer:

KH2PO4 2.31 g 0.17 M
K2HPO4 12.54 g 0.72 M
H2O to 100 ml

After autoclaving, and just before using the TB, add the KPO4 buffer to the TB medium.

 

 

Adjust to pH 7.0 with NaOH if necessary.