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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Repair of DNA ends for blunt ligation

- Set up the following reaction:

DNA 25 µl
5x T4 polymerase buffer 8 µl
10 mM dNTP mix 0.5 µl
100 mM ATP 0.5 µl
T4 polynucleotide kinase 1 µl
T4 polymerase 1 µl
H2O 2.5 µl


- Incubate at room temperature for 45 min.

- Inactivate for 10 minutes at 70°C