3' recessed end fill-in with DNA polymerase I, large (Klenow) fragment
- Dissolve DNA in 1X NEB restricion buffer.
- Supplement with 33µM dNTPs (or just use 1 µl of 10pmol/µl dNTPs).
- Add 1 unit Klenow per microgram of DNA (NEB enzyme is at 5 U/µl.
- Incubate at 25°C for 15 min.