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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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3' recessed end fill-in with DNA polymerase I, large (Klenow) fragment

  • Dissolve DNA in 1X NEB restricion buffer.
  • Supplement with 33µM dNTPs (or just use 1 µl of 10pmol/µl dNTPs).
  • Add 1 unit Klenow per microgram of DNA (NEB enzyme is at 5 U/µl.
  • Incubate at 25°C for 15 min.