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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Thawing tissue culture cells

  • Thaw a tube of frozen cells in a 37°C waterbath.
  • Transfer cells to a 15ml falcon tube with a pasteur pipette.
  • Add 1-2ml of medium (without Hepes) drop by drop, mixing after each drop.
  • Add medium to 10 ml.
  • Spin down cells, and plate in fresh medium in a small dish.