Creativity

Innovation

Originality

Imagination

 

Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Gap Repair

Gap repair is nearly identical to a regular transformation. Follow the protocol for regular transformations here, with the following change: In the master yeast mix, add linearized vector. 25 ng of linearized vector is sufficient, though up to 100 ng per transformation can be used to increase the efficiency. Linearize vector by enzymatic digestion, and heat inactivate the enzyme. Further cleanup is not needed.