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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Lithium Acetate Transformation Solutions

TE/LiAc

  • 1/10 volume 1M LiAc
  • 1/10 volume 10x TE (100mM Tris, 10mM EDTA, pH 8.0)
  • Fill up with H2O

PEG solutions (50% or 60%)

  • Dissolve PEG (MW 3,350) in water. This is easiest to do by first heating the water to ~80°C, and then slowly stirring in the PEG.
  • Filter sterilize (this will take a long time, but does work).

TE/LiAc/PEG

  • 1/10 volume 1M LiAc
  • 1/10 volume 10x TE (100mM Tris, 10mM EDTA, pH 8.0)
  • Fill up with 50% PEG [MW3,350] in H2O

Salmon sperm DNA

Various levels of purity and size of salmon sperm DNA are sold by Sigma and others. One of the cheapest is a high molecular weight DNA from Sigma (Deoxyribonucleic acid Sodium Salt Type III from Salmon Testes, Sigma D1626). According to the Gietz lab website, high molecular weight DNA works better, though I have not verified this. Dissolve the DNA in TE buffer at a concentration of 2 mg/ml (higher is not feasible due to the viscosity caused by the long DNA strands). The easiest is to dissolve it overnight in the cold room while stirring with a stir bar. Aliquot the DNA and store at -20°C. Before use, the DNA needs to be boiled for 5 min.