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Salient

Salient is an excellent design with a fresh approach for the ever-changing Web. Integrated with Gantry 5, it is infinitely customizable, incredibly powerful, and remarkably simple.

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Y2H controls

The following are the controls of known reporter activation strength that we use in the lab. Those for the Y strains were selected purely on their phenotype, hence the somewhat random collection of proteins used.

Controls for MaV103 and MaV203

 DB cloneAD clone
1 pPC97 -Cycloheximide pPC86
2 DB::Rb pocket AD::E2F (336-436)
3 DB::Fos HLH AD::Jun HLH
4 Gal4 (full length) pPC86

5

DB::DP1 (full length)

AD::E2F1 (157-436)

Controls for Y8800 and Y8900

 DB cloneAD clone-Leu -Trp -His-Leu -Trp -His
+ 2mM 3AT
-Leu -Trp -Ade-Leu -His
+Cycloheximide
1 pPC97 -Cyclo (empty vector) pPC86 (empty vector) 0 0 0 0
2 dpm-1 fars-1 2 1 0 3
3 mbk-1 Y79H2A.3a (1403 – 2130) 3 2 0 0
4 pas-6 pas-7 3 3 0 0
5 rfc-3 eif-3.D 3 3 1 3
6 Fos HLH Jun HLH 3 3 2 3

7

DP1 (full length)

E2F1 (157-436)

3 3 3 3