attL X attR reaction

 

Frame: ACA AGT TTG TAC AAA AAA GCA GGC T------AC CCA GCT TTC TTG TAC AAA GTG GT

Entry
clone

N75-ACAAGTTTGTACAAAAAAGCAGGCT------------------ACCCAGCTTTCTTGTACAAAGTGGT-N75
N75-TGTTCAAACATGTTTTTTCGTCCGA------------------TGGGTCGAAAGAACATGTTTCACCA-N75
[-----------attL1-----------]        ORF       [-----------attL2-----------]
 
                                     X
Destination
vector
ACAAGTTTGTACAAAAAAGCAGGCT-N100--------N100-ACCCAGCTTTCTTGTACAAAGTGGT
TGTTCAAACATGTTTTTTCGTCCGA-N100--------N100-TGGGTCGAAAGAACATGTTTCACCA
[-----------attR1------------]  ccdB  [-----------attR2------------]
 

                                     =

Expression
clone

ACAAGTTTGTACAAAAAAGCAGGCT------------------ACCCAGCTTTCTTGTACAAAGTGGT
TGTTCAAACATGTTTTTTCGTCCGA------------------TGGGTCGAAAGAACATGTTTCACCA
[--------attB1----------]        ORF       [--------attB2----------]

 
                                     +

By-
product

N75-ACAAGTTTGTACAAAAAAGCAGGCT-N100--------N100-ACCCAGCTTTCTTGTACAAAGTGGT-N75
N75-TGTTCAAACATGTTTTTTCGTCCGA-N100--------N100-TGGGTCGAAAGAACATGTTTCACCA-N75
[-------------attP1--------------]  ccdB  [-------------attP2--------------]