LR reaction

 

  1. Assemble the reaction:
   
5 µl
10 µl
  Entry clone (50-150ng)
x µl
x µl
  Destination vector
75 ng
150 ng
  5X LR Buffer
1 µl
2 µl
  TE pH 8.0
to 4 µl
to 8 µl
  LR Clonase
0.5µl
1 µl
  1. Incubate at 25°C for 1 hour to overnight.
  2. Transform into competent cells and plate on plates containing a suitable antibiotic.