att site structures and sequences

 

General structure of the recombination sites

att site structure

 

attB sequences

attB1 ACA AGT TTG TAC AAA AAA GCA GGC nnn nnn
attB1.1   ACA ACT TTG TAC AAA AAA GTT Gnn nnn nnn
attB2 nn nnC CCA GCT TTC TTG TAC AAA GTG GT
attB2.2 nn nnn nCA ACT TTC TTG TAC AAA GTT GT
attB4 CA ACT TTG TAT AGA AAA GTT Gnn nnn nnn

Gene specific sequences are indicated by 'n'.

 

Reading frames of the cassettes

Reading Frame A
ATC ACA AGT TTG TAC AAA AAA --- --- T TTC TTG TAC AAA GTG GTG AT
TAG TGT TCA AAC ATG TTT TTT --- --- A AAG AAC ATG TTT CAC CAC TA
Reading Frame B
A TCA ACA AGT TTG TAC AAA AAA --- --- T TTC TTG TAC AAA GTG GTT GAT
T AGT TGT TCA AAC ATG TTT TTT --- --- A AAG AAC ATG TTT CAC CAA CTA
Reading Frame C.1
NAT CAA ACA AGT TTG TAC AAA AAA --- --- T TTC TTG TAC AAA GTG GTT CGA T
NTA TAG TGT TCA AAC ATG TTT TTT --- --- A AAG AAC ATG TTT CAC CAA GCT A